Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

This Anti-Syntaxin 17 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of Syntaxin 17.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Syntaxin 17 in human kidney and human adrenal gland tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Syntaxin-17 (UniProt P56962) is encoded by the STX17 gene (Gene ID 55014) in human. Syntaxin-17 belongs to the Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) family of proteins that mediate membrane fusion events. Syntaxin-17 functions as the autophagosomal Qa-SNARE required for autophagosome-lysosome fusion and it mediates the fusion process by binding to its partner SNAREs, synaptosomal-associated protein 29 (SNAP29; Qbc-SNARE), and vesicle-associated membrane protein 8 (VAMP8; R-SNARE). In addition, the homotypic fusion and protein sorting (HOPS) complex is reported to interact with Syntaxin-17 and promotes Syntaxin-17-dependent autophagosome-lysosome fusion.

Immunogen

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Syntaxin 17.

Epitope: cytoplasmic domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human adrenal tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Syntaxin 17 in 10 µg of human adrenal tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~38 kDa observed. Uncharacterized band(s) may appear in some lysates.